Universal Multiplexable matK Primers for DNA Barcoding of Angiosperms
نویسندگان
چکیده
منابع مشابه
Universal multiplexable matK primers for DNA barcoding of angiosperms1
PREMISE OF THE STUDY PCR amplification of the matK barcoding region is often difficult when dealing with multiple angiosperm families. We developed a primer cocktail to amplify this region efficiently across angiosperm diversity. METHODS AND RESULTS We developed 14 matK primers (seven forward, seven reverse) for multiplex PCR, using sequences available in GenBank for 178 taxa belonging to 123...
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1.Atcheson, C.L., B. DiDomenico, S. Frackman, R.E. Esposito and R.T. Elder. 1987. Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene. Proc. Natl. Acad. Sci. USA 84:80358039. 2.Cha, J., W. Bishai and S. Chandrasegaran. 1993. New vectors for direct cloning of PCR products [published erratum appears in Gene 1994; 141:149]. Gene 136:369-370. 3.Clark, J.M. 19...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2016
ISSN: 2168-0450
DOI: 10.3732/apps.1500137